|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse IL17A ORF mammalian expression plasmid, C-GFPSpark tag||MG51065-ACG|
|Mouse IL17A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51065-ACR|
|Mouse IL17A ORF mammalian expression plasmid, C-Flag tag||MG51065-CF|
|Mouse IL17A ORF mammalian expression plasmid, C-His tag||MG51065-CH|
|Mouse IL17A ORF mammalian expression plasmid, C-Myc tag||MG51065-CM|
|Mouse IL17A ORF mammalian expression plasmid, C-HA tag||MG51065-CY|
|Mouse IL17A Gene cDNA clone plasmid||MG51065-G|
|Mouse IL17A ORF mammalian expression plasmid, N-Flag tag||MG51065-NF|
|Mouse IL17A ORF mammalian expression plasmid, N-His tag||MG51065-NH|
|Mouse IL17A ORF mammalian expression plasmid, N-Myc tag||MG51065-NM|
|Mouse IL17A ORF mammalian expression plasmid, N-HA tag||MG51065-NY|
|Mouse IL17A natural ORF mammalian expression plasmid||MG51065-UT|
IL17, also known as IL17a, is a cytokine belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.