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マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone in cloning vector

データシートレビュー関連製品プロトコル
Mouse GLRX cDNA クローン製品情報
Gene_bank_ref_id:NM_053108.4
cDNA サイズ:324bp
cDNA の説明:Full length Clone DNA of Mus musculus glutaredoxin.
遺伝子の別名:Grx1, Glrx1, TTase, C86710, D13Wsu156e
:Mouse
ベクター:PGEM-T Vector
Plasmid:pGEM-mGLRX
制限サイト:
タグ シーケンス:
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone in cloning vector on other vectors
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-GFPSpark タグMG52947-ACGJPY54410
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-OFPSpark タグMG52947-ACRJPY54410
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-GFPSpark タグMG52947-ANGJPY54410
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-OFPSpark タグMG52947-ANRJPY54410
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-Flag タグMG52947-CFJPY47150
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-His タグMG52947-CHJPY47150
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-Myc タグMG52947-CMJPY47150
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, C-HA タグMG52947-CYJPY47150
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone in cloning vectorMG52947-GJPY18140
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-Flag タグMG52947-NFJPY47150
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-His タグMG52947-NHJPY47150
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-Myc タグMG52947-NMJPY47150
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmid, N-HA タグMG52947-NYJPY47150
マウス glutaredoxin-1 / GRX1 / GLRX Gene ORF cDNA clone expression plasmidMG52947-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Glutaredoxin-1, also known as GRX1 and GLRX, belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. Glutaredoxin-1 functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. Glutaredoxin-1 exists in either a reduced or an oxidized form. Glutaredoxins function as electron carriers in the glutathione-dependent synthesis of deoxyribonucleotides by the enzymeribonucleotide reductase.

参考文献
  • Holmgren A. et al., 1988, FEMS Microbiol Rev. 4 (4): 271-97.
  • Holmgren A. 1988, Biochem Soc Trans. 16 (2): 95-6.
  • Holmgren A. 1989, J Biol Chem. 264 (24): 13963-6.
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    カタログ: MG52947-G
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