|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse GLO1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50364-ACG|
|Mouse GLO1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50364-ACR|
|Mouse GLO1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50364-ANG|
|Mouse GLO1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50364-ANR|
|Mouse GLO1 ORF mammalian expression plasmid, C-Flag tag||MG50364-CF|
|Mouse GLO1 ORF mammalian expression plasmid, C-His tag||MG50364-CH|
|Mouse GLO1 ORF mammalian expression plasmid, C-Myc tag||MG50364-CM|
|Mouse GLO1 ORF mammalian expression plasmid, C-HA tag||MG50364-CY|
|Mouse GLO1 Gene cDNA clone plasmid||MG50364-M|
|Mouse GLO1 ORF mammalian expression plasmid, N-Flag tag||MG50364-NF|
|Mouse GLO1 ORF mammalian expression plasmid, N-His tag||MG50364-NH|
|Mouse GLO1 ORF mammalian expression plasmid, N-Myc tag||MG50364-NM|
|Mouse GLO1 ORF mammalian expression plasmid, N-HA tag||MG50364-NY|
|Mouse GLO1 natural ORF mammalian expression plasmid||MG50364-UT|
Mouse Lactoylglutathione lyase, also known as Methylglyoxalase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, S-D-lactoylglutathione methylglyoxal lyase and GLO1, is a member of the glyoxalase I family. GLO1 / Glyoxalase I is a ubiquitous cellular defense enzyme involved in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Accumulative evidence suggests an important role of GLO1 expression in protection against methylglyoxal-dependent protein adduction and cellular damage associated with diabetes, cancer, and chronological aging. GLO1 / Glyoxalase I has been implicated in anxiety-like behavior in mice and in multiple psychiatric diseases in humans. GLO1 / Glyoxalase I catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. GLO1 / Glyoxalase I exists in three separable isoforms which originate from two alleles in the genome. These correspond to two homodimers and one heterodimer composed of two subunits showing different electrophoretic properties. GLO1 upregulation may play a functional role in glycolytic adaptations of cancer cells.