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マウス UDP galactose-4'-epimerase / GALE Gene ORF cDNA clone expression plasmid, C-HA タグ

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    マウス GALE cDNA クローン製品情報
    Gene_bank_ref_id:NM_178389.3
    cDNA サイズ:1044bp
    cDNA の説明:Full length Clone DNA of Mus musculus galactose-4-epimerase, UDP with C terminal HA tag.
    遺伝子の別名:AI323962, 2310002A12Rik
    :Mouse
    ベクター:pCMV3-C-HA
    Plasmid:
    制限サイト:
    タグ シーケンス:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    シーケンスの説明:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with GALE qPCR primers for gene expression analysis, MP201643 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    保存:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    マウス UDP galactose-4'-epimerase / GALE Gene ORF cDNA clone expression plasmid, C-HA タグ on other vectors
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    マウス UDP galactose-4'-epimerase / GALE Gene ORF cDNA clone expression plasmid, N-His タグMG51770-NHJPY47150
    マウス UDP galactose-4'-epimerase / GALE Gene ORF cDNA clone expression plasmid, N-Myc タグMG51770-NMJPY47150
    マウス UDP galactose-4'-epimerase / GALE Gene ORF cDNA clone expression plasmid, N-HA タグMG51770-NYJPY47150
    マウス UDP galactose-4'-epimerase / GALE Gene ORF cDNA clone expression plasmidMG51770-UTJPY47150
     発現ベクターの詳細情報
    Product nameProduct name
    背景

    UDP galactose-4'-epimerase, also known as GALE, enables the body to process a simple sugar called galactose, which is present in small amounts in many foods. Galactose is primarily part of a larger sugar called lactose, which is found in all dairy products and many baby formulas. UDP galactose-4'-epimerase catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. Defects in GALE causes epimerase-deficiency galactosemia (EDG), also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation.

    参考文献
  • Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
  • Lee KA. et al., 2011,. J Biol Chem. 286 (48): 41530-8.
  • McCorvie TJ. et al., 2012, Biochim Biophys Acta. 1822 (10): 1516-26.
  • Size / Price
    カタログ: MG51770-CY
    定価: 
    価格:      (You Save: )

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