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マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, C-Flag タグ

データシートレビュー関連製品プロトコル
Mouse F3 cDNA クローン製品情報
Gene_bank_ref_id:NM_010171.3
cDNA サイズ:885bp
cDNA の説明:Full length Clone DNA of Mus musculus coagulation factor III with C terminal Flag tag.
遺伝子の別名:TF, Cf3, Cf-3, CD142, AA409063, F3
:Mouse
ベクター:pCMV3-C-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, C-Flag タグ on other vectors
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグMG50413-ACGJPY54410
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグMG50413-ACRJPY54410
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, C-Flag タグMG50413-CFJPY47150
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, C-His タグMG50413-CHJPY47150
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, C-Myc タグMG50413-CMJPY47150
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, C-HA タグMG50413-CYJPY47150
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone in cloning vectorMG50413-MJPY18140
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, N-Flag タグMG50413-NFJPY47150
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, N-His タグMG50413-NHJPY47150
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, N-Myc タグMG50413-NMJPY47150
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmid, N-HA タグMG50413-NYJPY47150
マウス Coagulation Factor III / Tissue Factor / CD142 Gene ORF cDNA clone expression plasmidMG50413-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Tissue factor (TF), also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation; the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes. Coagulation Factor III is a transmembrane glycoprotein that localizes the coagulation serine protease factor VII/VIIa (FVII/VIIa) to the cell surface. The primary function of TF is to activate the clotting cascade. The TF:FVIIa complex also activates cells by cleavage of a G-protein coupled receptor called protease-activated receptor 2 (PAR2). TF is expressed by tumor cells and contributes to a variety of pathologic processes, such as thrombosis, metastasis, tumor growth, and tumor angiogenesis. As a key regulator of haemostasis and angiogenesis, it is also involved in the pathology of several diseases, including cardiovascular, inflammatory and neoplastic conditions.

参考文献
  • Morrissey JH. (2004) Tissue factor: a key molecule in hemostatic and nonhemostatic systems. Int J Hematol. 79(2): 103-8.
  • Milsom C, et al. (2008) Tissue factor and cancer. Pathophysiol Haemost Thromb. 36(3-4): 160-76.
  • Kasthuri RS, et al. (2009) Role of tissue factor in cancer. J Clin Oncol. 27(29): 4834-8.
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    カタログ: MG50413-CF
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