( We provide with ENTPD1 qPCR primers for gene expression analysis, MP200416 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|マウス CD39 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグ||MG50398-ACG|
|マウス CD39 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグ||MG50398-ACR|
|マウス CD39 Gene ORF cDNA clone expression plasmid, C-Flag タグ||MG50398-CF|
|マウス CD39 Gene ORF cDNA clone expression plasmid, C-His タグ||MG50398-CH|
|マウス CD39 Gene ORF cDNA clone expression plasmid, C-Myc タグ||MG50398-CM|
|マウス CD39 Gene ORF cDNA clone expression plasmid, C-HA タグ||MG50398-CY|
|マウス CD39 Gene ORF cDNA clone in cloning vector||MG50398-M|
|マウス CD39 Gene ORF cDNA clone expression plasmid, N-Flag タグ||MG50398-NF|
|マウス CD39 Gene ORF cDNA clone expression plasmid, N-His タグ||MG50398-NH|
|マウス CD39 Gene ORF cDNA clone expression plasmid, N-Myc タグ||MG50398-NM|
|マウス CD39 Gene ORF cDNA clone expression plasmid, N-HA タグ||MG50398-NY|
|マウス CD39 Gene ORF cDNA clone expression plasmid||MG50398-UT|
CD39, also known as ENTPD1, belongs to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. It is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 transgenic mice exhibit impaired platelet aggregation, prolonged bleeding times, and resistance to systemic thromboembolism. There is a correlation between ATP hydrolysis and triglycerides in patients with chronic heart disease, suggesting a relationship between ATP diphosphohydrolase and thrombogenesis. In the nervous system, CD39 could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.