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マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone in cloning vector

データシートレビュー関連製品プロトコル
Mouse ENO1 cDNA クローン製品情報
Gene_bank_ref_id:NM_023119.2
cDNA サイズ:1305bp
cDNA の説明:Full length Clone DNA of Mus musculus enolase 1, alpha non-neuron.
遺伝子の別名:Eno-1, MBP-1, AL022784, 0610008I15
:Mouse
ベクター:PGEM-T Vector
Plasmid:pGEM-mENO1
制限サイト:
タグ シーケンス:
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence except for the point mutation: 34G>A(E12K)
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone in cloning vector on other vectors
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-GFPSpark タグMG52810-ACGJPY54410
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-OFPSpark タグMG52810-ACRJPY54410
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-GFPSpark タグMG52810-ANGJPY54410
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-OFPSpark タグMG52810-ANRJPY54410
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-Flag タグMG52810-CFJPY47150
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-His タグMG52810-CHJPY47150
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-Myc タグMG52810-CMJPY47150
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-HA タグMG52810-CYJPY47150
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone in cloning vectorMG52810-GJPY18140
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-Flag タグMG52810-NFJPY47150
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-His タグMG52810-NHJPY47150
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-Myc タグMG52810-NMJPY47150
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-HA タグMG52810-NYJPY47150
マウス ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmidMG52810-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
参考文献
  • Capello M, et al. (2011) a-Enolase: a promising therapeutic and diagnostic tumor target. FEBS J. 278(7): 1064-74.
  • Kang HJ, et al. (2008) Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme. Acta Crystallogr D Biol Crystallogr. 64(Pt 6): 651-7.
  • Lopez-Alemany R, et al. (2005) Alpha-enolase plasminogen receptor in myogenesis. Front Biosci. 10: 30-6.
  • Ejeskdr K, et al. (2005) Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death. BMC Cancer. 5: 161.
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    カタログ: MG52810-G
    定価: 
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