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マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, N-Flag タグ

データシートレビュー関連製品プロトコル
Mouse CPE cDNA クローン製品情報
Gene_bank_ref_id:NM_013494.3
cDNA サイズ:1431bp
cDNA の説明:Full length Clone DNA of Mus musculus carboxypeptidase E with N terminal Flag tag.
遺伝子の別名:CPH; fat; Cph1; Cph-1; R74677
:Mouse
ベクター:pCMV3-N-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, N-Flag タグ on other vectors
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, C-GFPSpark タグMG51055-ACGJPY54410
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マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, N-GFPSpark タグMG51055-ANGJPY54410
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, N-OFPSpark タグMG51055-ANRJPY54410
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, C-Flag タグMG51055-CFJPY18140
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, C-His タグMG51055-CHJPY47150
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, C-Myc タグMG51055-CMJPY47150
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, C-HA タグMG51055-CYJPY47150
Mouse CPE Gene cDNA clone plasmidMG51055-GJPY18140
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, N-Flag タグMG51055-NFJPY47150
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, N-His タグMG51055-NHJPY47150
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, N-Myc タグMG51055-NMJPY47150
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmid, N-HA タグMG51055-NYJPY47150
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone in cloning vectorMG51055-UJPY18140
マウス Carboxypeptidase E/CPE Gene ORF cDNA clone expression plasmidMG51055-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Human carboxypeptidase E (CPE), also known as Carboxypeptidase H, is a peripheral membrane protein and a zinc metallocarboxypeptidase, and the conversion of proCPE into CPE occurs primarily in secretory vesicles. The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. The enzymatic activity is enhanced by millimolar concentrations of Co2+. It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway.Its interaction with glycosphingolipid-cholesterol rafts at the TGN facilitates the targeting. Mutations in this gene are implicated in type I I diabetes due to impaired glucose clearance and insulin resistance.

参考文献
  • Manser, E. et al., 1990, Biochem. J. 267: 517-525.
  • Cool, D.R. et al., 1997, Cell. 88: 73-83.
  • Song, L. and Fricker, L. 1995, J. Neurochem. 65: 444-453.
  • Dhanvantari,S. et al., 2000, J. Biol. Chem. 275: 29887-29893.
  • Jeffrey, K.D. et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105: 8452-8457
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