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マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid

データシートレビュー関連製品プロトコル
Mouse CKB cDNA クローン製品情報
Gene_bank_ref_id:NM_021273.4
cDNA サイズ:1146bp
cDNA の説明:Full length Clone DNA of Mus musculus creatine kinase, brain.
遺伝子の別名:Bck, Ck3, B-CK, Ck-3, Ckbb
:Mouse
ベクター:pCMV3-untagged
Plasmid:
制限サイト:
タグ シーケンス:
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid on other vectors
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, C-GFPSpark タグMG51386-ACGJPY54410
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, C-OFPSpark タグMG51386-ACRJPY54410
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, N-GFPSpark タグMG51386-ANGJPY54410
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, N-OFPSpark タグMG51386-ANRJPY54410
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, C-Flag タグMG51386-CFJPY47150
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, C-His タグMG51386-CHJPY47150
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, C-Myc タグMG51386-CMJPY47150
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, C-HA タグMG51386-CYJPY47150
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, N-Flag タグMG51386-NFJPY47150
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, N-His タグMG51386-NHJPY47150
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, N-Myc タグMG51386-NMJPY47150
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmid, N-HA タグMG51386-NYJPY47150
マウス CKB / Creatine kinase B type Gene ORF cDNA clone in cloning vectorMG51386-UJPY18140
マウス CKB / Creatine kinase B type Gene ORF cDNA clone expression plasmidMG51386-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

CKB(Creatine kinase B type) contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It belongs to the ATP:guanido phosphotransferase family. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CKB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. CKB reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer in brain, smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone etc.

参考文献
  • Wienker TF, et al. (1985) A dominant mutation causing ectopic expression of the creatine kinase B gene maps on chromosome 14. Cytogenet Cell Genet. 40:776.
  • Mariman EC, et al. (1989) Complete nucleotide sequence of the human creatine kinase B gene. Nucleic Acids Res. 17(15):6385.
  • Bong S, et al. (2008) Structural studies of human brain-type creatine kinase complexed with the ADP–Mg2+–NO3−–creatine transition-state analogue complex. FEBS Letters. 582(28): 3959-65.
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    カタログ: MG51386-UT
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