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マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-Myc タグ

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Mouse CDK2 cDNA クローン製品情報
Gene_bank_ref_id:NM_016756.4
cDNA サイズ:897bp
cDNA の説明:Full length Clone DNA of Mus musculus cyclin-dependent kinase 2, transcript variant 2 with N terminal Myc tag.
遺伝子の別名:A630093N05Rik, Cdk2
:Mouse
ベクター:pCMV3-N-Myc
Plasmid:
制限サイト:
タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-Myc タグ on other vectors
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マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグMG50796-ANGJPY54410
マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグMG50796-ANRJPY54410
マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-Flag タグMG50796-CFJPY47150
マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-His タグMG50796-CHJPY47150
マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-Myc タグMG50796-CMJPY47150
マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-HA タグMG50796-CYJPY47150
マウス CDK2 transcript variant 2 Gene ORF cDNA clone in cloning vectorMG50796-GJPY18140
マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-Flag タグMG50796-NFJPY47150
マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-His タグMG50796-NHJPY47150
マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-Myc タグMG50796-NMJPY47150
マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-HA タグMG50796-NYJPY47150
マウス CDK2 transcript variant 2 Gene ORF cDNA clone expression plasmidMG50796-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. Cdks (cyclin-dependent kinases) are heteromeric serine/threonine kinases that control progression through the cell cycle in concert with their regulatory subunits, the cyclins. Cdks are constitutively expressed and are regulated by several kinases and phosphastases, including Wee1, CDK-activating kinase and Cdc25 phosphatase. Although there are 12 different cdk genes, only 5 have been shown to directly drive the cell cycle (Cdk1, -2, -3, -4, and -6). Following extracellular mitogenic stimuli, cyclin D gene expression is upregulated. Cdk4 forms a complex with cyclin D and phosphorylates Rb protein, leading to liberation of the transcription factor E2F. E2F induces transcription of genes including cyclins A and E, DNA polymerase and thymidine kinase. Cdk4-cyclin E complexes form and initiate G1/S transition. Subsequently, Cdk1-cyclin B complexes form and induce G2/M phase transition. Cdk1-cyclin B activation induces the breakdown of the nuclear envelope and the initiation of mitosis. CDK2 associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. CDK2 is involved in the control of the cell cycle. It also interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.

参考文献
  • Bao ZQ, et al. (2011) Briefly bound to activate: transient binding of a second catalytic magnesium activates the structure and dynamics of CDK2 kinase for catalysis. Structure. 19(5):675-90.
  • Neganova I, et al. (2011) An important role for CDK2 in G1 to S checkpoint activation and DNA damage response in human embryonic stem cells. Stem Cells. 29(4):651-9.
  • Li J, et al. (2011) Phosphorylation of MCM3 protein by cyclin E/cyclin-dependent kinase 2 (Cdk2) regulates its function in cell cycle. J Biol Chem. 286(46):39776-85.
  • Buis J, et al. (2012) Mre11 regulates CtIP-dependent double-strand break repair by interaction with CDK2. Nat Struct Mol Biol. 19(2):246-52.
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