|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Mouse CD48 ORF mammalian expression plasmid, C-GFPSpark tag||MG50415-ACG|
|Mouse CD48 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50415-ACR|
|Mouse CD48 ORF mammalian expression plasmid, C-Flag tag||MG50415-CF|
|Mouse CD48 ORF mammalian expression plasmid, C-His tag||MG50415-CH|
|Mouse CD48 ORF mammalian expression plasmid, C-Myc tag||MG50415-CM|
|Mouse CD48 ORF mammalian expression plasmid, C-HA tag||MG50415-CY|
|Mouse CD48 Gene cDNA clone plasmid||MG50415-M|
|Mouse CD48 ORF mammalian expression plasmid, N-Flag tag||MG50415-NF|
|Mouse CD48 ORF mammalian expression plasmid, N-His tag||MG50415-NH|
|Mouse CD48 ORF mammalian expression plasmid, N-Myc tag||MG50415-NM|
|Mouse CD48 ORF mammalian expression plasmid, N-HA tag||MG50415-NY|
|Mouse CD48 natural ORF mammalian expression plasmid||MG50415-UT|
Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.