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マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone expression plasmid, C-Myc タグ

データシートレビュー関連製品プロトコル
Mouse CA4 cDNA クローン製品情報
Gene_bank_ref_id:NM_007607.2
cDNA サイズ:918bp
cDNA の説明:Full length Clone DNA of Mus musculus carbonic anhydrase 4 with C terminal Myc tag.
遺伝子の別名:Ca4, AW456718, Car4
:Mouse
ベクター:pCMV3-C-Myc
Plasmid:
制限サイト:
タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone expression plasmid, C-Myc タグ on other vectors
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マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone expression plasmid, C-His タグMG50350-CHJPY47151
マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone expression plasmid, C-Myc タグMG50350-CMJPY47151
マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone expression plasmid, C-HA タグMG50350-CYJPY47151
マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone in cloning vectorMG50350-MJPY18135
マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone expression plasmid, N-Flag タグMG50350-NFJPY47151
マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone expression plasmid, N-His タグMG50350-NHJPY47151
マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone expression plasmid, N-Myc タグMG50350-NMJPY47151
マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone expression plasmid, N-HA タグMG50350-NYJPY47151
マウス Carbonic Anhydrase IV / Car4 / CA4 Gene ORF cDNA clone expression plasmidMG50350-UTJPY47151
 発現ベクターの詳細情報
Product nameProduct name
背景

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

参考文献
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Baird TT, et al. (1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.
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    カタログ: MG50350-CM
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