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マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-Myc タグ

データシートレビュー関連製品プロトコル
Mouse AK4 cDNA クローン製品情報
Gene_bank_ref_id:NM_001177602.1
cDNA サイズ:672bp
cDNA の説明:Full length Clone DNA of Mus musculus adenylate kinase 4 with N terminal Myc tag.
遺伝子の別名:Ak3, AK 4, Ak-3, Ak-4, Ak3l1, D4Ertd274e
:Mouse
ベクター:pCMV3-N-Myc
Plasmid:
制限サイト:
タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-Myc タグ on other vectors
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグMG52601-ACGJPY54410
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグMG52601-ACRJPY54410
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグMG52601-ANGJPY54410
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグMG52601-ANRJPY54410
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-Flag タグMG52601-CFJPY47150
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-His タグMG52601-CHJPY47150
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-Myc タグMG52601-CMJPY47150
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-HA タグMG52601-CYJPY47150
Mouse AK4 Gene cDNA clone plasmidMG52601-GJPY18140
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-Flag タグMG52601-NFJPY47150
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-His タグMG52601-NHJPY47150
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-Myc タグMG52601-NMJPY47150
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-HA タグMG52601-NYJPY47150
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone in cloning vectorMG52601-UJPY18140
マウス AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmidMG52601-UTJPY47150
 発現ベクターの詳細情報
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背景

Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

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カタログ: MG52601-NM
定価: 
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