MERS-CoV (NCoV / Novel coronavirus) Spike Protein S2 Antibody, Rabbit PAb, Antigen Affinity Purified

1/1
Price:
Size:
Number:

MERS-CoV (NCoV / Novel coronavirus) Spike Protein S2 Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗体) の製品情報

製品名
MERS-CoV (NCoV / Novel coronavirus) Spike Protein S2 Antibody, Rabbit PAb, Antigen Affinity Purified
検証済のアプリケーション
WB,ELISA,IHC-P,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
アプリケーションの説明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特異性
MERS-CoV CoV spike
免疫原
Recombinant MERS-CoV (NCoV / Novel coronavirus) Spike glycoprotein Protein (Catalog#40070-V08B)
調製
Produced in rabbits immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Spike Protein S2 (Catalog#40070-V08B; AFS88936.1; Asp726-Pro1296), and purified by antigen affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

MERS-CoV (NCoV / Novel coronavirus) Spike Protein S2 Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

MERS-CoV (NCoV / Novel coronavirus) Spike Protein S2 Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗体) の画像

Anti-Novel coronavirus (HCoV-EMC/2012) Spike Protein S2 (aa 726-1296) rabbit polyclonal antibody at 1:1000 dilution

Sample:Novel coronavirus (HCoV-EMC/2012) Spike protein (ECD, aa 1-1297) 

Lane A: 5ng

Lane B: 1ng

Lane C: 0.5ng

Sample:Novel coronavirus (HCoV-EMC/2012) Spike Protein S2 (aa 726-1296) 

Lane D: 10ng

Lane E: 5ng

Lane F: 2ng

Secondary

Goat anti Rabbit IgG (H+L)/HRP at 0.4ug/ml..

Performed under reducing conditions.

MERS-CoV (NCoV / Novel coronavirus) Spike Protein S2 Antibody, Rabbit PAb, Antigen Affinity Purified: 別名

Anti-S Antibody

CoV spike 背景情報

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
参考文献
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください