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MERS-CoV (NCoV / Novel coronavirus) orf4b ORF mammalian expression plasmid, C-HA タグ

  • other Green fluorescent protein / GFP Gene Plasmid Map 5612
データシートレビュー関連製品プロトコル
MERS-CoV CoV-orf4b cDNA クローン製品情報
Gene_bank_ref_id:JX869059.2
cDNA サイズ:783 bp
cDNA の説明:Full length Clone DNA of MERS-CoV (NCoV / Novel coronavirus) orf4b with C terminal HA tag.
遺伝子の別名:CoV-orf4b
:MERS-CoV
ベクター:pCMV3-C-HA
Plasmid:pCMV3-NS3C-HA
制限サイト:KpnI + XbaI(6kb+0.78kb)
タグ シーケンス:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シーケンスの説明:Identical with the Gene Bank AFS88939.1.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at ambient temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

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カタログ: VG40084-CY
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