After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

クイック注文

Text Size:AAA

インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-His タグ

データシートレビュー関連製品プロトコル
H6N1 HA cDNA クローン製品情報
Gene_bank_ref_id:
cDNA サイズ:1701bp
cDNA の説明:Full length Clone DNA of Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) HA with N terminal His tag.
遺伝子の別名:HA1, Hemagglutinin
:H6N1
ベクター:pCMV3-SP-N-His
Plasmid:pCMV3-His-H6N1-NC-07-HA
制限サイト:KpnI + XbaI (6kb + 1.75kb)
タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シーケンスの説明:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with ACE81692.1.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at ambient temperature for three months.
H6N1 HA Gene Plasmid Map
Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-His タグ on other vectors
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG11723-ACGJPY83420
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP タグVG11723-ACRJPY83420
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized)VG11723-CJPY76170
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized), C-Flag タグVG11723-CFJPY76170
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, C-His タグVG11723-CHJPY76170
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, C-Myc タグVG11723-CMJPY76170
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, C-HA タグVG11723-CYJPY76170
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-Flag タグVG11723-NFJPY76170
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-His タグVG11723-NHJPY76170
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG11723-NMJPY76170
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-HA タグVG11723-NYJPY76170
インフルエンザ A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized)VG11723-UTJPY76170
 発現ベクターの詳細情報
Product nameProduct name
背景

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

参考文献
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37
  • Size / Price
    カタログ: VG11723-NH
    定価: 
    価格:      (You Save: )
    Contact Us
    画像
    • Influenza A H6N1 (A/northern shoveler/California/HKWF115/07) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-His tag
      最近閲覧したアイテム
        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        注意:すべての製品は、"研究目的のみに使用するものであり、診断または治療目的に使用するものではありません"