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インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA (Codon Optimized) ORF mammalian expression plasmid, N-His タグ

データシートレビュー関連製品プロトコル
H5N1 NA cDNA クローン製品情報
Gene_bank_ref_id:
cDNA サイズ:1350bp
cDNA の説明:Full length Clone DNA of Influenza A H5N1 (A/Thailand/1(KAN-1)/2004) Neuraminidase with N terminal His tag.
遺伝子の別名:Neuraminidase, NA
:H5N1
ベクター:pCMV3-SP-N-His
Plasmid:
制限サイト:
タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シーケンスの説明:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with AFF60789.1.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at ambient temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA (Codon Optimized) ORF mammalian expression plasmid, N-His タグ on other vectors
インフルエンザ A H5N1 (A/Thailand/1(KAN-1)/2004) Neuraminidase / NA ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG40064-ACGJPY78590
インフルエンザ A H5N1 (A/Thailand/1(KAN-1)/2004) Neuraminidase / NA (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP タグVG40064-ACRJPY78590
インフルエンザ A H5N1 (A/Thailand/1(KAN-1)/2004) Neuraminidase / NA ORF mammalian expression plasmid (Codon Optimized)VG40064-CJPY71330
インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA ORF mammalian expression plasmid (Codon Optimized), C-Flag タグVG40064-CFJPY71330
インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA (Codon Optimized) ORF mammalian expression plasmid, C-His タグVG40064-CHJPY71330
インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA (Codon Optimized) ORF mammalian expression plasmid, C-Myc タグVG40064-CMJPY71330
インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA (Codon Optimized) ORF mammalian expression plasmid, C-HA タグVG40064-CYJPY71330
インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA (Codon Optimized) ORF mammalian expression plasmid, N-Flag タグVG40064-NFJPY71330
インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA (Codon Optimized) ORF mammalian expression plasmid, N-His タグVG40064-NHJPY71330
インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG40064-NMJPY71330
インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA (Codon Optimized) ORF mammalian expression plasmid, N-HA タグVG40064-NYJPY71330
インフルエンザ A H5N1 (Thailand/1(KAN-1)/2004) Neuraminidase / NA ORF mammalian expression plasmid (Codon Optimized)VG40064-UTJPY71330
 発現ベクターの詳細情報
Product nameProduct name
背景

Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell.

Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our own cell surface proteins.

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.

Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. Monoclonal or polyclonal antibody can be raised with protein based antigen or peptide based antigen. Antibody raised with protein based antigen could have better specificity and/or binding affinity than antibody raised with peptide based antigen, but cost associated with the recombinant protein antigen is usually higher. Anti influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection.

Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.

参考文献
  • Sardet C., et al.,(1989), Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter. Cell 56:271-280.
  • Sardet C., et al., (1990), Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.Science 247:723-726.
  • Tse C.-M., et al.,(1991), Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.EMBO J. 10:1957-1967.
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    カタログ: VG40064-NH
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