クイック注文

インフルエンザ A H13N8 (A/black-headed gull/Netherlands/1/00) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-His タグ

    データシートレビュー関連製品プロトコル
    H13N8 HA cDNA クローン製品情報
    Gene_bank_ref_id:
    cDNA サイズ:1698bp
    cDNA の説明:Full length Clone DNA of Influenza A H13N8 (A/black-headed gull/Netherlands/1/00) HA with N terminal His tag.
    遺伝子の別名:HA1, Hemagglutinin
    :H13N8
    ベクター:pCMV3-SP-N-His
    Plasmid:
    制限サイト:
    タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
    シーケンスの説明:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with AAV91212.1.
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    保存:The lyophilized plasmid can be stored at ambient temperature for three months.
    His Tag Info

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

    Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

    インフルエンザ A H13N8 (A/black-headed gull/Netherlands/1/00) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-His タグ on other vectors
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    インフルエンザ A H13N8 (A/black-headed gull/Netherlands/1/00) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-Flag タグVG11721-NFJPY76170
    インフルエンザ A H13N8 (A/black-headed gull/Netherlands/1/00) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-His タグVG11721-NHJPY76170
    インフルエンザ A H13N8 (A/black-headed gull/Netherlands/1/00) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG11721-NMJPY76170
    インフルエンザ A H13N8 (A/black-headed gull/Netherlands/1/00) Hemagglutinin (Codon Optimized) ORF mammalian expression plasmid, N-HA タグVG11721-NYJPY76170
    インフルエンザ A H13N8 (A/black-headed gull/Netherlands/1/00) Hemagglutinin ORF mammalian expression plasmid (Codon Optimized)VG11721-UTJPY76170
     発現ベクターの詳細情報
    Product nameProduct name
    背景

    The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

    参考文献
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37
  • Size / Price
    カタログ: VG11721-NH
    定価: 
    価格:      (You Save: )

    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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