After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

クイック注文

Text Size:AAA

人々 CD122 / IL-2RB Gene ORF cDNA clone expression plasmid

データシートレビュー関連製品プロトコル
Human IL2RB/CD122 cDNA クローン製品情報
Gene_bank_ref_id:NM_000878.2
cDNA サイズ:1656bp
cDNA の説明:Full length Clone DNA of Homo sapiens interleukin 2 receptor, beta.
遺伝子の別名:IL2RB, CD122, P70-75
:Human
ベクター:pCMV/hygro
Plasmid:pCMV-IL2RB
制限サイト:KpnI + XbaI (5.5kb + 1.66kb)
タグ シーケンス:
シーケンスの説明:Identical with the Gene Bank Ref.ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Human IL2RB/CD122 Gene Plasmid Map
Human IL2Rb / CD122 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
pCMV/hygro Vector Information
 
Vector Name pCMV/hygro
Vector Size 5657bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag None
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)


Schematic of pCMV/hygro Multiple Cloning Sites
Product nameProduct name
背景

Interleukin-2 receptor (IL-2R) also known as High affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.

参考文献
  • Foss F. (2006) Clinical experience with denileukin diftitox (ONTAK). Semin Oncol. 33(1 Suppl 3): 11-6.
  • Sprent J, et al. (2001) T cell death and memory. Science. 293(5528): 245-8.
  • Teshigawara K, et al. (1987) Interleukin 2 high-affinity receptor expression requires two distinct binding proteins. J Exp Med. 165 (1): 223-38.
  • Size / Price
    カタログ: HG10696-M-N
    定価: 
    価格:      (You Save: )
    Contact Us
    画像
    • Human IL2Rb / CD122 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
      最近閲覧したアイテム
        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        注意:すべての製品は、"研究目的のみに使用するものであり、診断または治療目的に使用するものではありません"