Anti-IL1RA Antibody

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Anti-IL1RA Antibody (Rabbit Monoclonal 抗体) の製品情報

製品名
Anti-IL1RA Antibody
検証済のアプリケーション
FCM,ICC/IF
交差反応
Reacts with: Human
特異性
Human IL1RA
免疫原
Recombinant Human IL-1RA protein (Catalog#10123-HNAE)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Human IL-1RA (rh IL-1RA; Catalog#10123-HNAE; NP_776214.1; Arg26-Glu177).
ソース
Monoclonal Rabbit IgG Clone #004
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IL1RA Antibody (Rabbit Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IL1RA Antibody (Rabbit Monoclonal 抗体) の画像

Immunofluorescence staining of Human IL1Ra in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human IL1Ra monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human IL1RA expression on A431 cells. Cells were stained with purified anti-Human IL1RA, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-IL1RA Antibody: 別名

Anti-DIRA Antibody; Anti-ICIL-1RA Antibody; Anti-IL-1ra Antibody; Anti-IL-1ra3 Antibody; Anti-IL-1RN Antibody; Anti-IL1F3 Antibody; Anti-IL1RA Antibody; Anti-IRAP Antibody; Anti-MVCD4 Antibody

IL1RA 背景情報

Interleukin-1 receptor antagonist (IL-1RA) also known as IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of this protein encoding gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. IL-1RA/IL1RN may inhibit the activity of IL-1 by binding to its receptor and it has no IL-1 like activity. Genetic variation in IL-1RA/IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in IL-1RA/IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) which is also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis, and pustulosis from birth.
完全な名称
interleukin 1 receptor antagonist
参考文献
  • Langdahl BL, et al. (2000) Osteoporotic fractures are associated with an 86-base pair repeat polymorphism in the interleukin-1--receptor antagonist gene but not with polymorphisms in the interleukin-1beta gene. J Bone Miner. 15 (3): 402-14.
  • El-Omar EM, et al. (2000) Interleukin-1 polymorphisms associated with increased risk of gastric cancer. Nature. 404 (6776): 398-402.
  • Steinkasserer A, et al. (1992) The human IL-1 receptor antagonist gene (IL1RN) maps to chromosome 2q14-q21, in the region of the IL-1 alpha and IL-1 beta loci. Genomics. 13 (3): 654-7.

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