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人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグ

データシートレビュー関連製品プロトコル
Human USH1C cDNA クローン製品情報
Gene_bank_ref_id:NM_005709.2
cDNA サイズ:1659bp
cDNA の説明:Full length Clone DNA of Homo sapiens Usher syndrome 1C (autosomal recessive, severe), transcript variant 1 with C terminal Myc tag.
遺伝子の別名:PDZ73, AIE-75, DFNB18, PDZ-45, PDZ-73, NY-CO-37, NY-CO-38, ush1cpst, PDZ-73/NY-CO-38
:Human
ベクター:pCMV3-C-Myc
Plasmid:
制限サイト:
タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグ on other vectors
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG10613-ACGJPY59240
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG10613-ACRJPY59240
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG10613-ANGJPY59240
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG10613-ANRJPY59240
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10613-CFJPY51990
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His タグHG10613-CHJPY51990
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグHG10613-CMJPY51990
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA タグHG10613-CYJPY51990
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone in cloning vectorHG10613-MJPY18140
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10613-M-FJPY51990
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグHG10613-NFJPY51990
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグHG10613-NHJPY51990
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc タグHG10613-NMJPY51990
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA タグHG10613-NYJPY51990
人々 USH1C/Harmonin transcript variant 1 Gene ORF cDNA clone expression plasmidHG10613-UTJPY51990
 発現ベクターの詳細情報
Product nameProduct name
背景

Harmonin, also known as Antigen NY-CO-38 / NY-CO-37, Autoimmune enteropathy-related antigen AIE-75, Protein PDZ-73, Renal carcinoma antigen NY-REN-3, Usher syndrome type-1C protein and USH1C, is a protein which is expressed in small intestine, colon, kidney, eye and weakly in pancreas. USH1C is expressed also in vestibule of the inner ear. USH1C contains 3 PDZ (DHR) domains. USH1C may be involved in protein-protein interaction. Defects in USH1C are the cause of Usher syndrome type 1C (USH1C), also known as Usher syndrome type I Acadian variety. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). Defects in USH1C are also the cause of deafness autosomal recessive type 18 (DFNB18) which is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

参考文献
  • Verpy, E. et al., 2000, Nat Genet. 26 (1):51-5.
  • Weil D., et al., 2003, Hum. Mol. Genet. 12:463-471.
  • Reiners,J. et al., 2005, Hum Mol Genet. 14 (24):3933-43.
  • Yan,D. et al., 2006, Mol Biol. 357 (3):755-64.
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    カタログ: HG10613-CM
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