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人々 TRIM63 Gene ORF cDNA clone expression plasmid, C-Myc タグ

Human TRIM63 cDNA クローン製品情報
cDNA サイズ:1062bp
cDNA の説明:Full length Clone DNA of Homo sapiens tripartite motif containing 63, E3 ubiquitin protein ligase with C terminal Myc tag.
遺伝子の別名:IRF, SMRZ, MURF1, MURF2, RNF28, FLJ32380, TRIM63
制限サイト:KpnI + XbaI (6kb + 1.11kb)
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence.
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Human TRIM63 Gene Plasmid Map
Human TRIM63 ORF mammalian expression plasmid, C-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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カタログ: HG14146-CM
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  • Human TRIM63 ORF mammalian expression plasmid, C-Myc tag
  • other Green fluorescent protein / GFP Gene Plasmid Map 5611
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