( We provide with Spp1 qPCR primers for gene expression analysis, HP100389 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid, C-GFPSpark タグ||HG10352-ACG|
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid, C-OFPSpark タグ||HG10352-ACR|
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid, C-Flag タグ||HG10352-CF|
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid, C-His タグ||HG10352-CH|
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid, C-Myc タグ||HG10352-CM|
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid, C-HA タグ||HG10352-CY|
|人々 OPN/Osteopontin Gene ORF cDNA clone in cloning vector||HG10352-M|
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid, N-Flag タグ||HG10352-NF|
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid, N-His タグ||HG10352-NH|
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid, N-Myc タグ||HG10352-NM|
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid, N-HA タグ||HG10352-NY|
|人々 OPN/Osteopontin Gene ORF cDNA clone expression plasmid||HG10352-UT|
Osteopontin, also known as Secreted phosphoprotein 1, Bone sialoprotein 1, BSP-1, OPN, and SPP1, is a member of the osteopontin family and a SIBLING glycoprotein. Osteopontin has been classified as T-helper 1 cytokine and thus believed to exacerbate inflammation in several chronic inflammatory diseases, including atherosclerosis. Besides proinflammatory functions, physiologically Osteopontin is a potent inhibitor of mineralization, it prevents ectopic calcium deposits and is a potent inducible inhibitor of vascular calcification. Osteopontin is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. Osteopontin recruitment functions of inflammatory cells are thought to be mediated through its adhesive domains, especially the arginine-glycine-aspartate (RGD) sequence that interacts with several integrin heterodimers. Osteopontin has emerged as a potential biomarker and mediator in cardiovascular disease. In the context of atherosclerosis, OPN is generally regarded as a proinflammatory and proatherogenic molecule. However, the role of OPN in vascular calcification (VC), which is closely related to chronic and active inflammation, is that of a negative regulator because it is an inhibitor of calcification and an active inducer of decalcification. Extensive research has demonstrated the pivotal participation of Osteopontin in the regulation of cell signaling which controls neoplastic and malignant transformation. The elevated expression of Osteopontin has been observed in a variety of cancers. It has been linked with tumor metastasis and signifies a poor prognosis for the patient.