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Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, C-Flag tag

データシートレビュー関連製品プロトコル
Human DIABLO cDNA クローン製品情報
Gene_bank_ref_id:NM_019887.3
cDNA サイズ:720bp
cDNA の説明:Full length Clone DNA of Homo sapiens diablo homolog (Drosophila), nuclear gene encoding mitochondrial protein, transcript variant 1 with C terminal Flag tag.
遺伝子の別名:SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049
:Human
ベクター:pCMV3-C-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, C-Flag tag on other vectors
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, C-GFPSpark tagHG10339-ACGJPY54410
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, C-OFPSpark / RFP tagHG10339-ACRJPY54410
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, N-GFPSpark tagHG10339-ANGJPY54410
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, N-OFPSpark / RFP tagHG10339-ANRJPY54410
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, C-Flag tagHG10339-CFJPY47150
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, C-His tagHG10339-CHJPY47150
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, C-Myc tagHG10339-CMJPY47150
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, C-HA tagHG10339-CYJPY47150
Human SMAC / Diablo transcript variant 1 Gene cDNA clone plasmidHG10339-MJPY18140
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, Flag tagHG10339-M-FJPY47150
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, N-Flag tagHG10339-NFJPY47150
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, N-His tagHG10339-NHJPY47150
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, N-Myc tagHG10339-NMJPY47150
Human SMAC / Diablo transcript variant 1 ORF mammalian expression plasmid, N-HA tagHG10339-NYJPY47150
Human SMAC / Diablo transcript variant 1 natural ORF mammalian expression plasmidHG10339-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.

参考文献
  • Korga A, et al. (2006) Role of mitochondrial protein Smac/Diablo in regulation of apoptotic pathways Pol Merkur Lekarski. 20(119): 573-6.
  • Anguiano-Hernandez YM, et al. (2007) Smac/DIABLO and colon cancer. Anticancer Agents Med Chem. 7(4): 467-73.
  • Martinez-Ruiz G, et al. (2008) Role of Smac/DIABLO in cancer progression. J Exp Clin Cancer Res. 27: 48.
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    カタログ: HG10339-CF
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