Human SARS Coronavirus Spike Antibody, Rabbit PAb, Antigen Affinity Purified

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Human SARS Coronavirus Spike Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗体) の製品情報

製品名
Human SARS Coronavirus Spike Antibody, Rabbit PAb, Antigen Affinity Purified
検証済のアプリケーション
WB,ELISA,FCM,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
アプリケーションの説明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特異性
SARS CoV spike
免疫原
Recombinant Human SARS Coronavirus Spike Protein (Catalog#40150-V08B2)
調製
Produced in rabbits immunized with purified, recombinant Human SARS Coronavirus Spike ( Catalog#40150-V08B2; AAX16192.1; Arg306-Phe527). Human SARS Coronavirus Spike specific IgG was purified by Human SARS Coronavirus Spike affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human SARS Coronavirus Spike Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Human SARS Coronavirus Spike Antibody, Rabbit PAb, Antigen Affinity Purified (Rabbit Polyclonal 抗体) の画像

Anti-Human SARS Coronavirus Spike rabbit polyclonal antibody at 1:1000 dilution.

Sample:Human SARS Coronavirus Spike Recombinant Protein

Lane A: 50ng

Lane B: 10ng

Secondary

Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Human SARS Coronavirus Spike Antibody, Rabbit PAb, Antigen Affinity Purified: 別名

Anti-S1 Antibody

CoV spike 背景情報

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.
参考文献
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

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