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人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグ

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Human RAD17 cDNA クローン製品情報
Gene_bank_ref_id:NM_133338.1
cDNA サイズ:2013bp
cDNA の説明:Full length Clone DNA of Homo sapiens RAD17 homolog (S. pombe) , transcript variant 1 with N terminal Flag tag.
遺伝子の別名:CCYC, R24L, RAD24, HRAD17, RAD17SP, FLJ41520, RAD17
:Human
ベクター:pCMV3-N-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグ on other vectors
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG10546-ACGJPY59241
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人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG10546-ANGJPY59241
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG10546-ANRJPY59241
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10546-CFJPY51987
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His タグHG10546-CHJPY51987
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグHG10546-CMJPY51987
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA タグHG10546-CYJPY51987
人々 Rad17 transcript variant 1 Gene ORF cDNA clone in cloning vectorHG10546-MJPY18135
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10546-M-FJPY51987
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグHG10546-NFJPY51987
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグHG10546-NHJPY51987
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc タグHG10546-NMJPY51987
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA タグHG10546-NYJPY51987
人々 Rad17 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10546-UTJPY51987
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カタログ: HG10546-NF
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