|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|人々 Renin Gene ORF cDNA clone expression plasmid, C-GFPSpark タグ||HG10969-ACG|
|人々 Renin Gene ORF cDNA clone expression plasmid, C-OFPSpark タグ||HG10969-ACR|
|人々 Renin Gene ORF cDNA clone expression plasmid, C-Flag タグ||HG10969-CF|
|人々 Renin Gene ORF cDNA clone expression plasmid, C-His タグ||HG10969-CH|
|人々 Renin Gene ORF cDNA clone expression plasmid, C-Myc タグ||HG10969-CM|
|人々 Renin Gene ORF cDNA clone expression plasmid, C-HA タグ||HG10969-CY|
|人々 Renin Gene ORF cDNA clone in cloning vector||HG10969-M|
|人々 Renin Gene ORF cDNA clone expression plasmid||HG10969-M-N|
|人々 Renin Gene ORF cDNA clone expression plasmid, N-Flag タグ||HG10969-NF|
|人々 Renin Gene ORF cDNA clone expression plasmid, N-His タグ||HG10969-NH|
|人々 Renin Gene ORF cDNA clone expression plasmid, N-Myc タグ||HG10969-NM|
|人々 Renin Gene ORF cDNA clone expression plasmid, N-HA タグ||HG10969-NY|
|人々 Renin Gene ORF cDNA clone expression plasmid||HG10969-UT|
Mouse Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of the peptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.