クイック注文

人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-Flag タグ

データシートレビュー関連製品プロトコル
Human PMVK cDNA クローン製品情報
Gene_bank_ref_id:BC006089
cDNA サイズ:579bp
cDNA の説明:Full length Clone DNA of Homo sapiens phosphomevalonate kinase with N terminal Flag tag.
遺伝子の別名:PMK, PMKA, PMKASE, HUMPMKI
:Human
ベクター:pCMV3-N-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-Flag タグ on other vectors
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG14583-ACGJPY54410
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG14583-ACRJPY54410
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG14583-ANGJPY54410
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG14583-ANRJPY54410
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-Flag タグHG14583-CFJPY47150
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-His タグHG14583-CHJPY47150
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-Myc タグHG14583-CMJPY47150
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, C-HA タグHG14583-CYJPY47150
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone in cloning vectorHG14583-GJPY18140
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-Flag タグHG14583-NFJPY47150
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-His タグHG14583-NHJPY47150
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-Myc タグHG14583-NMJPY47150
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmid, N-HA タグHG14583-NYJPY47150
人々 PMVK / phosphomevalonate kinase Gene ORF cDNA clone expression plasmidHG14583-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

PMVK is a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of PMVK is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis.

Size / Price
カタログ: HG14583-NF
定価: 
価格:      (You Save: )
Contact Us
画像
      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      注意:すべての製品は、"研究目的のみに使用するものであり、診断または治療目的に使用するものではありません"