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人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, N-HA タグ

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Human PMM2 cDNA クローン製品情報
Gene_bank_ref_id:BC008310
cDNA サイズ:741bp
cDNA の説明:Full length Clone DNA of Homo sapiens phosphomannomutase 2 with N terminal HA tag.
遺伝子の別名:PMI, CDG1, CDGS, PMI1, CDG1a, PMM 2
:Human
ベクター:pCMV3-N-HA
Plasmid:
制限サイト:
タグ シーケンス:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, N-HA タグ on other vectors
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG14648-ACGJPY54410
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG14648-ACRJPY54410
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG14648-ANGJPY54410
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG14648-ANRJPY54410
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, C-Flag タグHG14648-CFJPY47150
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, C-His タグHG14648-CHJPY47150
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, C-Myc タグHG14648-CMJPY47150
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, C-HA タグHG14648-CYJPY47150
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone in cloning vectorHG14648-GJPY18140
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, N-Flag タグHG14648-NFJPY47150
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, N-His タグHG14648-NHJPY47150
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, N-Myc タグHG14648-NMJPY47150
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmid, N-HA タグHG14648-NYJPY47150
人々 phosphomannomutase 2 / PMM2 / CDG1 Gene ORF cDNA clone expression plasmidHG14648-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Phosphomannomutase 2, also known as PMM2 and CDG1, belongs to the eukaryotic PMM family. Phosphomannomutase 2 catalyzes the isomerization of mannose 6-phosphate to mannose 1-phosphate. Mannose 1-phosphate is a precursor to GDP-mannose necessary for the synthesis of dolichol-P-oligosaccharides. GDP-mannose can transfer its small sugar molecule called mannose to the growing oligosaccharide chain. Once the correct number of small sugar molecules are linked together to form the oligosaccharide, it can be attached to a protein. Phosphomannomutase 2 is also required for a number of critical mannosyl transfer reactions. Mutations in PMM2 gene have been shown to cause defects in the protein glycosylation pathway manifest as carbohydrate-deficient glycoprotein syndrome type I.

参考文献
  • Jaeken J. et al., 2002, Annual review of genomics and human genetics. 2: 129-51.
  • Matthijs G. et al., 2000, Mol Genet Metab. 68 (2): 220-6.
  • Matthijs G. et al., 1997, Nat Genet. 16 (1): 88-92.
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