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人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグ

データシートレビュー関連製品プロトコル
Human NPM1 cDNA クローン製品情報
Gene_bank_ref_id:NM_002520
cDNA サイズ:885bp
cDNA の説明:Full length Clone DNA of Homo sapiens nucleophosmin (nucleolar phosphoprotein B23, numatrin) with N terminal Flag tag.
遺伝子の別名:B23, NPM
:Human
ベクター:pCMV3-N-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグ on other vectors
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG12074-ACGJPY54410
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG12074-ACRJPY54410
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG12074-ANGJPY54410
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG12074-ANRJPY54410
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグHG12074-CFJPY47150
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His タグHG12074-CHJPY47150
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグHG12074-CMJPY47150
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA タグHG12074-CYJPY47150
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone in cloning vectorHG12074-GJPY18140
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグHG12074-NFJPY47150
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグHG12074-NHJPY47150
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc タグHG12074-NMJPY47150
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA タグHG12074-NYJPY47150
人々 NPM1/Nucleophosmin transcript variant 1 Gene ORF cDNA clone expression plasmidHG12074-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Nucleophosmin 1 (NPM1), also known as nucleolar phosphoprotein B23 or numatrin, is a member of the nucleoplasmin family. Nucleophosmin (NPM) is a nucleolar phosphoprotein that plays multiple roles in ribosome assembly and transport, cytoplasmic-nuclear trafficking, centrosome duplication and regulation of p53. The NPM1 gene is frequently involved in chromosomal translocation, mutation and deletion. Mutations of the NPM1 gene leading to the expression of a cytoplasmic mutant protein, NPMc+, are the most frequent genetic abnormalities found in acute myeloid leukemias. Acute myeloid leukemias (AML) with mutated NPM1 have distinct characteristics, including a significant association with a normal karyotype, involvement of different hematopoietic lineages, a specific gene-expression profile and clinically, a better response to induction therapy and a favorable prognosis. In addition, NPM1 is a crucial gene to consider in the context of the genetics and biology of cancer. NPM1 is frequently overexpressed, mutated, rearranged and deleted in human cancer. Traditionally regarded as a tumour marker and a putative proto-oncogene, it has now also been attributed with tumour-suppressor functions.

参考文献
  • Chen W, et al. (2006) Nucleophosmin gene mutations in acute myeloid leukemia. Arch Pathol Lab Med. 130(11): 1687-92.
  • Naoe T, et al. (2006) Nucleophosmin: a versatile molecule associated with hematological malignancies. Cancer Sci. 97(10): 963-9.
  • Grisendi S, et al. (2006) Nucleophosmin and cancer. Nat Rev Cancer. 6(7): 493-505.
  • Falini B, et al. (2007) Acute myeloid leukemia carrying cytoplasmic/mutated nucleophosmin (NPMc+ AML): biologic and clinical features. Blood. 109(3): 874-85.
  • Meani N, et al. (2009) Role of nucleophosmin in acute myeloid leukemia. Expert Rev Anticancer Ther. 9(9): 1283-94.
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    カタログ: HG12074-NF
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