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人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid

データシートレビュー関連製品プロトコル
Human NEK3 cDNA クローン製品情報
Gene_bank_ref_id:NM_152720.1
cDNA サイズ:1521bp
cDNA の説明:Full length Clone DNA of Homo sapiens NIMA (never in mitosis gene a)-related kinase 3, transcript variant 2.
遺伝子の別名:HSPK36, MGC29949, NEK3
:Human
ベクター:pCMV3-untagged
Plasmid:pCMV3-NEK3
制限サイト:KpnI + XbaI (6.1kb + 1.52kb)
タグ シーケンス:
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence except for the point mutation 1236T/G not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Human NEK3 Gene Plasmid Map
Human NEK3 transcript variant 2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid on other vectors
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG10675-ACGJPY59240
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG10675-ACRJPY59240
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG10675-ANGJPY59240
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG10675-ANRJPY59240
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10675-CFJPY51990
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-His タグHG10675-CHJPY51990
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-Myc タグHG10675-CMJPY51990
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-HA タグHG10675-CYJPY51990
人々 NEK3 transcript variant 2 Gene ORF cDNA clone in cloning vectorHG10675-MJPY18140
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10675-M-FJPY51990
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-Flag タグHG10675-NFJPY51990
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-His タグHG10675-NHJPY51990
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-Myc タグHG10675-NMJPY51990
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmid, N-HA タグHG10675-NYJPY51990
人々 NEK3 transcript variant 2 Gene ORF cDNA clone expression plasmidHG10675-UTJPY51990
 発現ベクターの詳細情報
Product nameProduct name
背景

NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. Members of the NEK family of protein kinases share high amino acid homology with NIMA (never in mitosis gene a). NEK3 differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. It is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. NEK3 mRNA can be detected in all the proliferating cell lines with the amount not changing during the cell cycle. Prolactin stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Multiple transcript variants encoding different isoforms have been found for NEK3 gene. NEK3 may play a role in mitotic regulation.

参考文献
  • Miller SL, et al. (2007) Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Oncogene. 26(32):4668-78.
  • Hernandez M, et al. (2006) Is there any association between nek3 and cancers with frequent 13q14 deletion?. Cancer Invest. 24(7):682-8.
  • Tanaka K, et al. (1999) Cloning and characterization of the murine Nek3 protein kinase, a novel member of the NIMA family of putative cell cycle regulators. J Biol Chem. 274(19):13491-7.
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    カタログ: HG10675-UT
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    • Human NEK3 transcript variant 2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
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