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人々 MST4/MST-4 Gene ORF cDNA clone expression plasmid

    データシートレビュー関連製品プロトコル
    人々 MST4 cDNA クローン製品情報
    Gene_bank_ref_id:NM_016542.3
    cDNA サイズ:1251bp
    cDNA の説明:Full length Clone DNA of Homo sapiens serine/threonine protein kinase MST4.
    遺伝子の別名:MST4, MASK
    :Human
    ベクター:pCMV3-untagged
    Plasmid:
    制限サイト:
    タグ シーケンス:
    シーケンスの説明:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with MST4 qPCR primers for gene expression analysis, HP100634 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    保存:The lyophilized plasmid can be stored at room temperature for three months.
    人々 MST4/MST-4 Gene ORF cDNA clone expression plasmid on other vectors
    Product nameProduct name
    背景

    MST4, also known as mammalian STE20-like protein kinase 4, is a novel member of the germinal center kinase subfamily of human Ste20-like kinases and is closely related to MST3. The 416 amino acid full-length MST4 contains a C-terminal regulatory domain and an N-terminal kinase domain, both of which are required for full activation of the kinase. MST4 is highly expressed in placenta, thymus, and peripheral blood leukocytes. MST4 specifically activates ERK but not JNK or p38 MAPK in transient transfected cells or in stable cell lines, and thus is biologically active in the activation of MEK/ERK pathway mediating cell growth and transformation. Further, MST4 kinase activity is stimulated significantly by epidermal growth factor receptor (EGFR) ligands, which are known to promote growth of certain cancer cells. Accordingly, MST4 have a potential role in signal transduction pathways involved in cancer progression. Three alternatively spliced isoform of MST4 have been isolated, and isoform 3 lacks an exon encoding kinase domain and may function as a dominant-negative regulator of the MST4 kinase.

    参考文献

    1. Qian, Z. et al., 2001, J Biol Chem. 276 :22439-45.

    2. Lin, JL. et al., 2001, Oncogene. 20: 6559-6569.

    3. Sung V, et al., 2003, Cancer research. 63: 3356-63.

    4. Ma, X. et al., 2007, Molecular biology of the cell. 18:1965-78.

    5. ten Klooster JP, et al., 2009, Developmental cell. 16:551-62.

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