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人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-Myc タグ

データシートレビュー関連製品プロトコル
Human MAPK8 cDNA クローン製品情報
Gene_bank_ref_id:NM_139047.1
cDNA サイズ:1284bp
cDNA の説明:Full length Clone DNA of Homo sapiens mitogen-activated protein kinase 8, transcript variant JNK1-b2 with N terminal Myc tag.
遺伝子の別名:JNK, JNK1, PRKM8, SAPK1, JNK1A2, JNK21B1/2
:Human
ベクター:pCMV3-N-Myc
Plasmid:
制限サイト:
タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-Myc タグ on other vectors
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人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG10795-ANGJPY54410
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG10795-ANRJPY54410
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10795-CFJPY47150
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-His タグHG10795-CHJPY47150
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-Myc タグHG10795-CMJPY47150
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-HA タグHG10795-CYJPY47150
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone in cloning vectorHG10795-MJPY18140
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10795-M-FJPY47150
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmidHG10795-M-NJPY47150
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-Flag タグHG10795-NFJPY47150
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-His タグHG10795-NHJPY47150
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-Myc タグHG10795-NMJPY47150
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-HA タグHG10795-NYJPY47150
人々 JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmidHG10795-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Mitogen-activated protein kinase 8 (MAPK8), also known as JNK1, is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The protein kinases JNK1 has been found to serve as critical molecular links between obesity, metabolic inflammation, and disorders of glucose homeostasis. It is critically involved in the promotion of diet-induced obesity, metabolic inflammation and beta-cell dysfunction. The selective deficiency of JNK1 in the murine nervous system is sufficient to suppress diet-induced obesity. Genetic analysis indicates that the effects of JNK1 can be separated from effects of JNK1 on obesity. JNK1 is a potential pharmacological target for the development of drugs that might be useful for the treatment of metabolic syndrome, and type 2 diabetes. Furthermore, JNK1 plays a major role in the hypoxic cellular damage. JNK1 protein might be an attractive target for antihypoxic therapy in increasing resistance to many pathological conditions and diseases, leading to the oxygen deficit.

参考文献
  • Betigeri S, et al. (2006) JNK1 as a molecular target to limit cellular mortality under hypoxia. Mol Pharm. 3(4): 424-30.
  • Solinas G, et al. (2010) JNK1 and IKKbeta: molecular links between obesity and metabolic dysfunction. FASEB J. 24(8): 2596-611.
  • Sabio G, et al. (2010) Role of the hypothalamic-pituitary-thyroid axis in metabolic regulation by JNK1. Genes Dev. 24(3): 256-64.
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