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人々 Lipopolysaccharide binding 蛋白質/LBP Gene ORF cDNA clone expression plasmid, N-HA タグ

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Human LBP cDNA クローン製品情報
Gene_bank_ref_id:NM_004139.2
cDNA サイズ:1446bp
cDNA の説明:Full length Clone DNA of Homo sapiens lipopolysaccharide binding protein with N terminal HA tag.
遺伝子の別名:MGC22233
:Human
ベクター:pCMV3-SP-N-HA
Plasmid:
制限サイト:
タグ シーケンス:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

人々 Lipopolysaccharide binding 蛋白質/LBP Gene ORF cDNA clone expression plasmid, N-HA タグ on other vectors
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人々 Lipopolysaccharide binding 蛋白質/LBP Gene ORF cDNA clone expression plasmid, N-Myc タグHG10526-NMJPY47150
人々 Lipopolysaccharide binding 蛋白質/LBP Gene ORF cDNA clone expression plasmid, N-HA タグHG10526-NYJPY47150
人々 Lipopolysaccharide binding 蛋白質/LBP Gene ORF cDNA clone expression plasmidHG10526-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Lipopolysaccharide binding protein ( LBP ) is a glycoprotein that is synthesized principally by hepatocytes. LBP is a trace plasma protein that binds to the lipid A moiety of bacterial lipopolysaccharides ( LPSs ). LBP binds directly to the outer membrane of Gram-negative bacteria and to purified aggregates of extracted endotoxin, and catalyses the delivery of endotoxin to membrane ( mCD14,GPI-Linked ) and soluble ( sCD14 ) forms of CD14, thereby markedly increasing host cell sensitivity to endotoxin. LBP efficiently catalyses the transfer of individual molecules of endotoxin to (s)CD14 only when LBP–endotoxin aggregates are formed in the presence of albumin. In the presence of EDTA, LBP binding promotes further disaggregation of endotoxin. LBP binding does not have such drastic effects under more physiological conditions, but may still induce more subtle topological rearrangements of endotoxin.

参考文献
  • J. Weiss, 2003, Biochemical Society Transactions: Volume 31, part 4: 785-790.
  • RR Schumann, et al., Science, 1990, Vol 249, Issue 4975: 1429-143.
  • Carsten J. Kirschning, et al., 1997, Genomics, Volume 46, Issue 3: 416-25.
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    カタログ: HG10526-NY
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