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人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグ

データシートレビュー関連製品プロトコル
Human IDS cDNA クローン製品情報
Gene_bank_ref_id:NM_000202.5
cDNA サイズ:1653bp
cDNA の説明:Full length Clone DNA of Homo sapiens iduronate 2-sulfatase, transcript variant 1 with C terminal Flag tag.
遺伝子の別名:MPS2, SIDS
:Human
ベクター:pCMV3-C-FLAG
Plasmid:pCMV3-IDS-Flag
制限サイト:KpnI + XbaI (6kb + 1.69kb)
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Human IDS Gene Plasmid Map
Human IDS / MPS2 transcript variant 1 natural ORF mammalian expression plasmid, C-Flag tag
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグ on other vectors
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG10337-ACGJPY59241
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG10337-ACRJPY59241
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10337-CFJPY51987
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His タグHG10337-CHJPY51987
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグHG10337-CMJPY51987
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA タグHG10337-CYJPY51987
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone in cloning vectorHG10337-MJPY18135
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグHG10337-NFJPY51987
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグHG10337-NHJPY51987
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc タグHG10337-NMJPY51987
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA タグHG10337-NYJPY51987
人々 Iduronate 2-Sulfatase / IDS transcript variant 1 Gene ORF cDNA clone expression plasmidHG10337-UTJPY51987
 発現ベクターの詳細情報
Product nameProduct name
背景

Iduronate 2-Sulfatase, also known as IDS, is a member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O- and N-sulfate esters from a variety of substrates. The human Iduronate 2-Sulfatase/IDS consists of a signal peptide, a pro peptide and a mature chain that may be further processed into two chains. Among the identified 18 human sulfatases, Iduronate 2-Sulfatase/IDS is required for the lysosomal degradation of the glycosaminoglycans (GAG), heparan sulfate and dermatan sulfate. Multiple mutations in this X-chromosome localized gene result in Iduronate 2-Sulfatase/IDS enzymatic deficiency, and lead to the sex-linked Mucopolysaccharidosis Type II (MPS II ), also known as Hunter Syndrome characterized by the lysosomal accumulation of the GAG and their excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe due to the level of Iduronate 2-Sulfatase/IDS enzyme. Retroviral-mediated Iduronate 2-Sulfatase/IDS gene transfer into lymphoid cells would be a promising gene therapeutic strategy.

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カタログ: HG10337-CF
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  • Human IDS / MPS2 transcript variant 1 natural ORF mammalian expression plasmid, C-Flag tag
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