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人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, C-Flag タグ

データシートレビュー関連製品プロトコル
Human IDO1 cDNA クローン製品情報
Gene_bank_ref_id:NM_002164.4
cDNA サイズ:1212bp
cDNA の説明:Full length Clone DNA of Homo sapiens indoleamine 2,3-dioxygenase 1 with C terminal Flag tag.
遺伝子の別名:IDO, INDO, IDO1
:Human
ベクター:pCMV3-C-FLAG
Plasmid:pCMV3-IDO1-Flag
制限サイト:KpnI + XbaI (6kb + 1.25kb)
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Human IDO1 Gene Plasmid Map
Human IDO1 natural ORF mammalian expression plasmid, C-Flag tag
Human IDO1 Gene Expression validated Image
Human IDO1 ORF mammalian expression plasmid, C-Flag tag
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Caption:
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, C-Flag タグ on other vectors
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG11650-ACGJPY54410
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG11650-ACRJPY54410
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG11650-ANGJPY54410
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG11650-ANRJPY54410
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, C-Flag タグHG11650-CFJPY47150
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, C-His タグHG11650-CHJPY47150
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, C-Myc タグHG11650-CMJPY47150
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, C-HA タグHG11650-CYJPY47150
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone in cloning vectorHG11650-MJPY18140
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, N-Flag タグHG11650-NFJPY47150
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, N-His タグHG11650-NHJPY47150
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, N-Myc タグHG11650-NMJPY47150
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmid, N-HA タグHG11650-NYJPY47150
人々 IDO1/Indoleamine 2,3‑dioxygenase Gene ORF cDNA clone expression plasmidHG11650-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Indoleamine 2,3-dioxygenase-1, also known as Indoleamine-pyrrole 2,3-dioxygenase, IDO1 and IDO, is a member of the indoleamine 2,3-dioxygenase family. IDO1 / IDO and tryptophan 2,3-dioxygenase (TDO) are tryptophan-degrading enzymes that catalyze the first step in tryptophan catabolism via the kynurenine pathway. TDO is widely distributed in both eukaryotes and bacteria. In contrast, IDO has been found only in mammals and yeast. In 2007, a third enzyme, indoleamine 2,3-dioxygenase-2 (IDO2), was discovered. IDO2 is found not only in mammals but also in lower vertebrates. IDO1 / IDO is an immunosuppressive molecule inducible in various cells. IDO1 / IDO catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. It mediates oxidative cleavage of tryptophan, an amino acid essential for cell proliferation and survival. IDO1 / IDO inhibition is proposed to have therapeutic potential in immunodeficiency-associated abnormalities, including cancer. The IDO pathway is activated in multiple tumor types. Selective inhibition of IDO1 may represent an attractive cancer therapeutic strategy via up-regulation of cellular immunity. IDO1 / IDO is an enzyme that suppresses adaptive T-cell immunity by catabolizing tryptophan from the cellular microenvironment. Inhibition of IDO pathway might enhance the efficacy of immunotherapeutic strategies for cancer.

参考文献
  • Barnes NA. et al., 2009, J Immunol. 183 (9): 5768-77.
  • Yuasa HJ. et al., 2009, Comp Biochem Physiol B Biochem Mol Biol. 153 (2): 137-44.
  • L b S. et al., 2009, Cancer Immunol Immunother. 58 (1): 153-7.
  • Liu,X. et al., 2010, Blood.115 (17): 3520-30.
  • Sun,T. et al., 2010, Mol Cell Biochem. 342 (1-2): 29-34.
  • Kiank C. et al., 2010, PLoS One. 5 (7): e11825.
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    カタログ: HG11650-CF
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    • Human IDO1 natural ORF mammalian expression plasmid, C-Flag tag
    • Human IDO1 ORF mammalian expression plasmid, C-Flag tag
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