Insulysin/insulin-degrading enzyme cDNA ORF Clone, Human, N-His tag General Information
Gene
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human insulin-degrading enzyme with N terminal His tag.
Plasmid
Promoter
Enhanced CMV promoter
Restriction Sites
KpnI(two restriction sites) + XbaI(6kb+3.06kb+0.06kb)
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
Insulysin/insulin-degrading enzyme cDNA ORF Clone, Human, N-His tag Validated Images
Insulysin/insulin-degrading enzyme cDNA ORF Clone, Human, N-His tag Alternative Names
INSULYSIN cDNA ORF Clone, Human
Insulysin/insulin-degrading enzyme Background Information
Full Name
insulin-degrading enzyme
