HK3 cDNA ORF Clone, Human, N-His tag

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HK3 cDNA ORF Clone, Human, N-His tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
2772 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence.
製品の特徴
Full length Clone DNA of Human hexokinase 3 (white cell) with N terminal His tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI (6kb + 2.82kb)
タグシーケンス
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

HK3 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

HK3 cDNA ORF Clone, Human, N-His tag: 検証済の画像

HK3 cDNA ORF Clone, Human, N-His tag: 別名

HKIII cDNA ORF Clone, Human; HXK3 cDNA ORF Clone, Human

HK3 背景情報

Hexokinase-3, also known as Hexokinase type III, HKIII and HK3, is a protein which belongs to thehexokinase family. Hexokinase-3 / HK3 is anenzymewhich in humans is encoded by theHK2gene. Hexokinases phosphorylate glucoseto produceglucose 6-phosphate, committing glucose to theglycolytic pathway. In mammalian tissues hexokinase exists as four isoenzymes encoded by distinct genes. These proteins are homologous and are organized in two homologous domains, with the exception of hexokinase type IV which has only one. This organization is believed to be the result of a duplication and tandem fusion event involving the gene encoding for the ancestral hexokinase. The gene encodes hexokinase-3. Similar to hexokinases-1 and hexokinases-2, thisallostericenzyme is inhibited by its product glucose 6-phosphate.
完全な名称
hexokinase 3 (white cell)
参考文献
  • Palma F. et al., 1996, Mol Cell Biochem. 155: 23-9.
  • Furuta H. et al.,1996, Genomics. 36 (1): 206-9.
  • Colosimo A. et al.,1996, Cytogenet Cell Genet. 74 (3): 187-8.
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