GM-CSF ELISA Kit, Human

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GM-CSF ELISA Kit, Human: 製品情報

製品名
GM-CSF ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Cell supernatant
感度
2.04 pg/mL
線形範囲
7.81-500 pg/mL
特異性
Recognizes both recombinant and natural Human GM-CSF
干渉
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human GM-CSF control were assayed for interference. No significant interference was observed.
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 74.52 152.95 304.79
SD 2.25 3.11 9.15
CV(%) 3.0% 2.0% 3.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 77.80 157.37 311.78
SD 3.74 8.52 12.78
CV(%) 4.8% 5.4% 4.1%
回収率
The recovery of Human CSF2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
101
Range
98-103%
直線性
Supernatant
  Recovery of detected
1:2 103%
1:4 97%
1:8 74%
1:16 72%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
製品概要
This GM-CSF ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human GM-CSF protein in Cell supernatant . It contains recombinant Human GM-CSF, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

GM-CSF ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human GM-CSF. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

GM-CSF ELISA Kit, Human: Alternative Names

CSF2 ELISA Kit, Human; GM-CSF ELISA Kit, Human; GMCSF ELISA Kit, Human

GM-CSF Background Information

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is one of an array of cytokines with pivotal roles in embryo implantation and subsequent development. Several cell lineages in the reproductive tract and gestational tissues synthesise GM-CSF under direction by ovarian steroid hormones and signalling agents originating in male seminal fluid and the conceptus. The pre-implantation embryo, invading placental trophoblast cells and the abundant populations of leukocytes controlling maternal immune tolerance are all subject to GM-CSF regulation. GM-CSF stimulates the differentiation of hematopoietic progenitors to monocytes and neutrophils, and reduces the risk for febrile neutropenia in cancer patients. GM-CSF also has been shown to induce the differentiation of myeloid dendritic cells (DCs) that promote the development of T-helper type 1 (cellular) immune responses in cognate T cells. The active form of the protein is found extracellularly as a homodimer, and the encoding gene is localized to a related gene cluster at chromosome region 5q31 which is known to be associated with 5q-syndrome and acute myelogenous leukemia. As a part of the immune/inflammatory cascade, GM-CSF promotes Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity, and thus worthy of consideration for therapeutic target. GM-CSF has been utilized in the clinical management of multiple disease processes. Most recently, GM-CSF has been incorporated into the treatment of malignancies as a sole therapy, as well as a vaccine adjuvant. While the benefits of GM-CSF in this arena have been promising, recent reports have suggested the potential for GM-CSF to induce immune suppression and, thus, negatively impact outcomes in the management of cancer patients. GM-CSF deficiency in pregnancy adversely impacts fetal and placental development, as well as progeny viability and growth after birth, highlighting this cytokine as a central maternal determinant of pregnancy outcome with clinical relevance in human fertility.
Full Name
colony stimulating factor 2 (granulocyte-macrophage)
References
  • Robertson SA. (2007) GM-CSF regulation of embryo development and pregnancy. Cytokine Growth Factor Rev. 18(3-4): 287-98.
  • Waller EK. (2007) The role of sargramostim (rhGM-CSF) as immunotherapy. Oncologist. 12 Suppl 2: 22-6.
  • Clive KS, et al. (2010) Use of GM-CSF as an adjuvant with cancer vaccines: beneficial or detrimental? Expert Rev Vaccines. 9(5): 519-25.
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