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人々 GBA/glucocerebrosidase Gene ORF cDNA clone expression plasmid, C-His タグ

  • Human GBA/glucocerebrosidase Gene Plasmid Map 17462
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人々 GBA cDNA クローン製品情報
Gene_bank_ref_id:NM_000157
cDNA サイズ:1656 bp
cDNA の説明:Full length Clone DNA of Homo sapiens glucosidase, beta, acid with C terminal His tag.
遺伝子の別名:GBA1
:Human
ベクター:pCMV3-C-His
Plasmid:pCMV3-GBA-His
制限サイト:HindIII(two restriction sites) + XbaI(6kb+1.53kb+0.15kb)
タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with GBA qPCR primers for gene expression analysis, HP101572 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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カタログ: HG12038-CH
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