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人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, N-Flag タグ

データシートレビュー関連製品プロトコル
Human FUOM cDNA クローン製品情報
Gene_bank_ref_id:NM_001098483.1
cDNA サイズ:465bp
cDNA の説明:Full length Clone DNA of Homo sapiens fucose mutarotase with N terminal Flag tag.
遺伝子の別名:FUCU, FucM, C10orf125
:Human
ベクター:pCMV3-N-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, N-Flag タグ on other vectors
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG13974-ACGJPY54410
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG13974-ACRJPY54410
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG13974-ANGJPY54410
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG13974-ANRJPY54410
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, C-Flag タグHG13974-CFJPY47150
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, C-His タグHG13974-CHJPY47150
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, C-Myc タグHG13974-CMJPY47150
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, C-HA タグHG13974-CYJPY47150
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone in cloning vectorHG13974-GJPY18140
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, N-Flag タグHG13974-NFJPY47150
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, N-His タグHG13974-NHJPY47150
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, N-Myc タグHG13974-NMJPY47150
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmid, N-HA タグHG13974-NYJPY47150
人々 FUOM / fucose mutarotase / FucM Gene ORF cDNA clone expression plasmidHG13974-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

FUOM, also known as fucose mutarotase and FucM, belongs to the RbsD / FucU family. FUOM is involved in the interconversion between alpha- and beta-L-fucoses. L-Fucose has two isforms: alpha-L-fucose (29.5%) and beta-L-fucose (70.5%). The beta-form is metabolized through the salvage pathway. GDP-L-fucose formed either by the de novo or salvage pathways is transported into the endoplasmic reticulum, where it serves as a substrate for N- and O-glycosylations by fucosyltransferases. Fucosylated structures expressed on cell surfaces or secreted in biological fluids are believed to play a critical role in cell-cell adhesion and recognition processes. FUOM mainly exists as homodimer, but also functions as homotetramer, homooctamer, and homodecamer. FUOM's homodimeric form seems catalytically inactive.

参考文献
  • Deloukas P. et al., 2004, Nature. 429: 375-81.
  • Ota T. et al., 2004, Nat Genet. 36: 40-5.
  • Dongkyu Park. et al., 2007, Glycobiology. 17 (9): 955-62.
  • Size / Price
    カタログ: HG13974-NF
    定価: 
    価格:      (You Save: )
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