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人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, C-Flag タグ

データシートレビュー関連製品プロトコル
Human FHIT cDNA クローン製品情報
Gene_bank_ref_id:BC032336
cDNA サイズ:444bp
cDNA の説明:Full length Clone DNA of Homo sapiens fragile histidine triad with C terminal Flag tag.
遺伝子の別名:FRA3B, AP3Aase
:Human
ベクター:pCMV3-C-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, C-Flag タグ on other vectors
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG14434-ACGJPY54405
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG14434-ACRJPY54405
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG14434-ANGJPY54405
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG14434-ANRJPY54405
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, C-Flag タグHG14434-CFJPY47151
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, C-His タグHG14434-CHJPY47151
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, C-Myc タグHG14434-CMJPY47151
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, C-HA タグHG14434-CYJPY47151
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone in cloning vectorHG14434-GJPY18135
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, N-Flag タグHG14434-NFJPY47151
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, N-His タグHG14434-NHJPY47151
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, N-Myc タグHG14434-NMJPY47151
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmid, N-HA タグHG14434-NYJPY47151
人々 Fragile histidine triad / FHIT Gene ORF cDNA clone expression plasmidHG14434-UTJPY47151
 発現ベクターの詳細情報
Product nameProduct name
背景

Fragile histidine triad, also known as FHIT, may play a key role in differentiating humans from apes. Fragile histidine triad gene belongs to the histidine triad gene family. It has been shown that fragile histidine triad synergizes with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. Fragile histidine triad gene works as a tumor suppressor as it has been demonstrated in animal studies. The exact molecular function of FHIT is still partially unclear. It also acts as a tumor suppressor of HER2/neu driven breast cancer.

参考文献
  • Lambert N, et al. (2006) An RNA gene expressed during cortical development evolved rapidly in humans. Nature. 443(7108):167-72.
  • Pekarsky Y, et al. (1998) Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans. Proc Natl Acad Sci. 95(15):8744-9.
  • Ohta M, et al. (1996) The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell. 84(4): 587-97.
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    カタログ: HG14434-CF
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