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人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, C-His タグ

データシートレビュー関連製品プロトコル
Human FAP cDNA クローン製品情報
Gene_bank_ref_id:NM_004460.2
cDNA サイズ:2283bp
cDNA の説明:Full length Clone DNA of Homo sapiens fibroblast activation protein, alpha with C terminal His tag.
遺伝子の別名:FAPA, DPPIV, DKFZp686G13158, FAP
:Human
ベクター:pCMV3-C-His
Plasmid:
制限サイト:
タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, C-His タグ on other vectors
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG10464-ACGJPY59240
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人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, C-Flag タグHG10464-CFJPY51990
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, C-His タグHG10464-CHJPY51990
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, C-Myc タグHG10464-CMJPY51990
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, C-HA タグHG10464-CYJPY51990
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone in cloning vectorHG10464-MJPY18140
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, C-His タグHG10464-M-HJPY51990
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, N-Flag タグHG10464-NFJPY51990
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, N-His タグHG10464-NHJPY51990
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, N-Myc タグHG10464-NMJPY51990
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmid, N-HA タグHG10464-NYJPY51990
人々 Fibroblast Activation 蛋白質 alpha/FAP Gene ORF cDNA clone expression plasmidHG10464-UTJPY51990
 発現ベクターの詳細情報
Product nameProduct name
背景

Seprase, also known as 170 kDa melanoma membrane-bound gelatinase , Fibroblast activation protein alpha, Integral membrane serine protease and FAP, is a single-pass type II membrane protein which belongs to the peptidase S9B family. Seprase / FAP is found in cell surface lamellipodia, invadopodia and on shed vesicles. Seprase / FAP appears to act as a proteolytically active 170-kDa dimer, consisting of two 97-kDa subunits. It is a member of the group type II integral serine proteases, which includes dipeptidyl peptidase IV ( DPPIV / CD26 ) and related type II transmembrane prolyl serine peptidases, which exert their mechanisms of action on the cell surface. Seprase / FAP colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Seprase / FAP colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. DPP4 and seprase exhibit multiple functions due to their abilities to form complexes with each other and to interact with other membrane-associated molecules. In association with DPP4, Seprase / FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. Seprase / FAP has a dual function in tumour progression. The proteolytic activity of Seprase has been shown to promote cell invasiveness towards the ECM and also to support tumour growth and proliferation. Seprase / FAP may have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.

参考文献
  • Mori,Y. et al., 2004, Oncology. 67 (5-6):411-9.
  • Aertgeerts K., et al., 2005, J. Biol. Chem. 280:19441-19444.
  • Liu T., et al., 2005, J. Proteome Res. 4:2070-2080.
  • Ghersi G., et al., 2006, Cancer Res. 66:4652-4661.
  • O'Brien,P. et al., 2008, Biochim Biophys Acta. 1784 (9):1130-45.
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    カタログ: HG10464-CH
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