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人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, N-Flag タグ

データシートレビュー関連製品プロトコル
Human DDC cDNA クローン製品情報
Gene_bank_ref_id:NM_000790.3
cDNA サイズ:1443bp
cDNA の説明:Full length Clone DNA of Homo sapiens dopa decarboxylase (aromatic L-amino acid decarboxylase) with N terminal Flag tag.
遺伝子の別名:AADC
:Human
ベクター:pCMV3-N-FLAG
Plasmid:pCMV3-Flag-DDC
制限サイト:HindIII + XbaI (6kb + 1.48kb)
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Human DDC Gene Plasmid Map
Human DDC natural ORF mammalian expression plasmid, N-Flag tag
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, N-Flag タグ on other vectors
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG10560-ACGJPY54410
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG10560-ACRJPY54410
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG10560-ANGJPY54410
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG10560-ANRJPY54410
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, C-Flag タグHG10560-CFJPY47150
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, C-His タグHG10560-CHJPY47150
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, C-Myc タグHG10560-CMJPY47150
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, C-HA タグHG10560-CYJPY47150
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone in cloning vectorHG10560-MJPY18140
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmidHG10560-M-NJPY47150
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, N-Flag タグHG10560-NFJPY47150
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, N-His タグHG10560-NHJPY47150
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, N-Myc タグHG10560-NMJPY47150
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmid, N-HA タグHG10560-NYJPY47150
人々 DOPA Decarboxylase/DDC Gene ORF cDNA clone expression plasmidHG10560-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.

参考文献
  • Ichinose, H. et al.,1989,Biochem. Biophys. Res. Commun. 164: 1024-1030.
  • Lisa, J. S. et al., 1992, Genomics 13: 469-471.
  • Moore, P. S. et al.,1996, Biochem. J. 315:249-256.
  • Bertoldi, M. et al., 2003, Biochim. Biophys. Acta. 1647:42-47.
  • Vassilacopoulou, D. et al., 2004, Neurochem. Res. 29: 1817-1823.
  • Ma, J.Z., et al., 2005, Hum. Mol. Genet. 14: 1691-1698.
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    カタログ: HG10560-NF
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    • Human DDC natural ORF mammalian expression plasmid, N-Flag tag
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