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人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, C-HA タグ

データシートレビュー関連製品プロトコル
Human DBH cDNA クローン製品情報
Gene_bank_ref_id:BC017174
cDNA サイズ:1812bp
cDNA の説明:Full length Clone DNA of Homo sapiens dopamine beta-hydroxylase (dopamine beta-monooxyge) with C terminal HA tag.
遺伝子の別名:DBM, DBH
:Human
ベクター:pCMV3-C-HA
Plasmid:
制限サイト:
タグ シーケンス:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, C-HA タグ on other vectors
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG13440-ACGJPY59240
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG13440-ACRJPY59240
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG13440-ANGJPY59240
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG13440-ANRJPY59240
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, C-Flag タグHG13440-CFJPY51990
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, C-His タグHG13440-CHJPY51990
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, C-Myc タグHG13440-CMJPY51990
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, C-HA タグHG13440-CYJPY51990
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone in cloning vectorHG13440-GJPY18140
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, N-Flag タグHG13440-NFJPY51990
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, N-His タグHG13440-NHJPY51990
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, N-Myc タグHG13440-NMJPY51990
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmid, N-HA タグHG13440-NYJPY51990
人々 DBH / Dopamine beta-Hydroxylase Gene ORF cDNA clone expression plasmidHG13440-UTJPY51990
 発現ベクターの詳細情報
Product nameProduct name
背景

DBH is a 290 kDa copper-containing oxygenase. It can be detected in noradrenergic nerve terminals of the central and peripheral nervous systems, and is also expressed in chromaffin cells of the adrenal medulla. DBH contains our identical subunits, and its activity requires ascorbate as a cofactor. It functions in in the synthesis of small-molecule neurotransmitters that is membrane-bound, making norepinephrine the only transmitter synthesized inside vesicles. DBH has been shown to be associated with decision making and addictive behaviors such as alcohol and smoking, attention deficit hyperactivity disorder, and also with neurological diseases such as Schizophrenia and Alzheimer's.

参考文献
  • Rush RA. et al., 1980, Crit Rev Clin Lab Sci. 12 (3): 241-77.
  • Goldstein M. et al., 1964, Life Sci. 3 (7): 763-7.
  • S Friedman. et al., 1966, The Journal of Biological Chemistry. 241 (10): 2256-9.
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    カタログ: HG13440-CY
    定価: 
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