CTHRC1 cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG11647-CF
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All CTHRC1 Reagents
CRO Services
Product recommended

All CTHRC1 Reagents
CTHRC1 cDNA ORF Clone, Human, C-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
771 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human collagen triple helix repeat containing 1 with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Restriction Sites
KpnI + XbaI(6kb+0.77kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
CTHRC1 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CTHRC1 cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
other Green fluorescent protein / GFP Gene Plasmid Map 5609
CTHRC1 Background Information

Collagen triple helix repeat-containing protein 1, also known as Protein NMTC1, and CTHRC1, is a secreted protein that is glycosylated and highly conserved from lower chordates to mammals. CTHRC1 expression was not detectable in normal arteries. However, it is transiently expressed in the arterial wall in response to injury where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration. A short collagen motif with 12 Gly-X-Y repeats appears to be responsible for trimerization of the CTHRC1 protein and this renders the molecule susceptible to cleavage by collagenase. CTHRC1 overexpression caused a dramatic reduction in collagen type I mRNA and protein levels. Currently available data indicate that Cthrc1 expression in vascular cells regulates transforming growth factor beta responsiveness, thereby impacting transforming growth factor beta target genes, including collagens. Additionally, CTHRC1 increases bone mass as a positive regulator of osteoblastic bone formation and offers an anabolic approach for the treatment of osteoporosis.

Full Name
collagen triple helix repeat containing 1
References
  • Pyagay P, et al. (2005) Collagen triple helix repeat containing 1, a novel secreted protein in injured and diseased arteries, inhibits collagen expression and promotes cell migration. Circ Res. 96(2): 261-8.
  • Durmus T, et al. (2006) Expression analysis of the novel gene collagen triple helix repeat containing-1 (Cthrc1). Gene Expr Patterns. 6(8): 935-40.
  • LeClair R, et al. (2007) The role of collagen triple helix repeat containing 1 in injured arteries, collagen expression, and transforming growth factor beta signaling. Trends Cardiovasc Med. 17(6): 202-5.
  • Kimura H, et al. (2008) Cthrc1 is a positive regulator of osteoblastic bone formation. PLoS One. 3(9): e3174.
  • CTHRC1 cDNA ORF Clone, Rat, Related Products

    Product Name Catalog#(PDF)
    CTHRC1 cDNA ORF Clone in Cloning Vector, Rat RG8A6878-U
    CTHRC1 cDNA ORF Clone in Cloning Vector, Rat RG80712-U
    CTHRC1 cDNA ORF Clone, Rat, untagged RG80712-UT
    CTHRC1 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80712-ACGLN
    CTHRC1 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80712-ACG
    CTHRC1 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG8A6878-ACGLN
    CTHRC1 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80712-ACR
    CTHRC1 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80712-CF
    CTHRC1 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80712-NF
    CTHRC1 cDNA ORF Clone, Rat, C-His tag RG80712-CH
    CTHRC1 cDNA ORF Clone, Rat, N-His tag RG80712-NH
    CTHRC1 cDNA ORF Clone, Rat, C-HA tag RG80712-CY
    CTHRC1 cDNA ORF Clone, Rat, N-HA tag RG80712-NY
    CTHRC1 cDNA ORF Clone, Rat, C-Myc tag RG80712-CM
    CTHRC1 cDNA ORF Clone, Rat, N-Myc tag RG80712-NM
    CTHRC1 qPCR Primer Pairs, Rat RP300676

    CTHRC1 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    CTHRC1 cDNA ORF Clone in Cloning Vector, Mouse MG50950-G
    CTHRC1 cDNA ORF Clone, Mouse, untagged MG50950-UT
    CTHRC1 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50950-ACGLN
    CTHRC1 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50950-ACG
    CTHRC1 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50950-ACR
    CTHRC1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50950-CF
    CTHRC1 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50950-NF
    CTHRC1 cDNA ORF Clone, Mouse, C-His tag MG50950-CH
    CTHRC1 cDNA ORF Clone, Mouse, N-His tag MG50950-NH
    CTHRC1 cDNA ORF Clone, Mouse, C-HA tag MG50950-CY
    CTHRC1 cDNA ORF Clone, Mouse, N-HA tag MG50950-NY
    CTHRC1 cDNA ORF Clone, Mouse, C-Myc tag MG50950-CM
    CTHRC1 cDNA ORF Clone, Mouse, N-Myc tag MG50950-NM
    CTHRC1 qPCR Primer Pairs, Mouse MP200919

    CTHRC1 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    CTHRC1 cDNA ORF Clone in Cloning Vector, Human HG11647-M
    CTHRC1 cDNA ORF Clone, Human, untagged HG11647-UT
    CTHRC1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11647-ACGLN
    CTHRC1 cDNA ORF Clone, Human, C-GFPSpark® tag HG11647-ACG
    CTHRC1 cDNA ORF Clone, Human, C-OFPSpark® tag HG11647-ACR
    CTHRC1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11647-NF
    CTHRC1 cDNA ORF Clone, Human, C-His tag HG11647-CH
    CTHRC1 cDNA ORF Clone, Human, N-His tag HG11647-NH
    CTHRC1 cDNA ORF Clone, Human, C-HA tag HG11647-CY
    CTHRC1 cDNA ORF Clone, Human, N-HA tag HG11647-NY
    CTHRC1 cDNA ORF Clone, Human, C-Myc tag HG11647-CM
    CTHRC1 cDNA ORF Clone, Human, N-Myc tag HG11647-NM
    CTHRC1 qPCR Primer Pairs, Human HP101495
    info info
    添加购物车成功! 添加购物车失败!