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人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグ

データシートレビュー関連製品プロトコル
Human CDK1 cDNA クローン製品情報
Gene_bank_ref_id:NM_001786.4
cDNA サイズ:894bp
cDNA の説明:Full length Clone DNA of Homo sapiens cell division cycle 2, G1 to S and G2 to M transcript variant 1 with N terminal His tag.
遺伝子の別名:CDK1, CDC28A, MGC111195, DKFZp686L20222, CDC2
:Human
ベクター:pCMV3-N-His
Plasmid:
制限サイト:
タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグ on other vectors
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人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG10739-ANGJPY54410
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人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグHG10739-CMJPY47150
人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA タグHG10739-CYJPY47150
人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone in cloning vectorHG10739-MJPY18140
人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10739-M-FJPY47150
人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10739-M-NJPY47150
人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグHG10739-NFJPY47150
人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグHG10739-NHJPY47150
人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc タグHG10739-NMJPY47150
人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA タグHG10739-NYJPY47150
人々 CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10739-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.

参考文献
  • Lee MG, et al. (1987) Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature. 327(6117):31-5.
  • Enserink JM, et al. (2010) An overview of Cdk1-controlled targets and processes. Cell Division. 5(11): 1-41.
  • Ninomiya-Tsuji J, et al. (1991) Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation. Proc Natl Acad Sci. 88(20):9006-10.
  • Zhan Q, et al. (1999) Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45. Oncogene. 18(18):2892-900.
  • Jin S, et al. (2000) The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression. J Biol Chem. 275(22):16602-8.
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