B7-H3 Protein, Human, Recombinant (His Tag), Biotinylated

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B7-H3 Protein, Human, Recombinant (His Tag), Biotinylated: 製品情報

純度
> 97 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the human CD276 (Q5ZPR3-1) extracellular domain (Met1-Thr461) was expressed, with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
NCBI Reference No.
発現ホスト
HEK293 Cells
Human
予測N末端
Leu 29
分子量
The recombinant human CD276 consists of 444 amino acids and predictes a molecular mass of 48 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD276 is approximately 73 kDa due to glycosylation.
バッファー
Lyophilized from a 0.2μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

B7-H3 Protein, Human, Recombinant (His Tag), Biotinylated: 画像

B7-H3 Protein, Human, Recombinant (His Tag), Biotinylated: 別名

4Ig-B7-H3 Protein, Human; B7-H3 Protein, Human; B7H3 Protein, Human; B7RP-2 Protein, Human

B7-H3 背景情報

B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.
氏名
CD276 molecule
参考文献
  • Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73.
  • Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8.
  • Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875.
  • Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12.
  • Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.
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