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人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, N-Flag タグ

データシートレビュー関連製品プロトコル
Human ENPEP cDNA クローン製品情報
Gene_bank_ref_id:NM_001977.2
cDNA サイズ:2874bp
cDNA の説明:Full length Clone DNA of Homo sapiens glutamyl aminopeptidase (aminopeptidase A) with N terminal Flag tag.
遺伝子の別名:APA, CD249, gp160
:Human
ベクター:pCMV3-SP-N-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, N-Flag タグ on other vectors
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG10554-ACGJPY78585
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG10554-ACRJPY78585
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, C-Flag タグHG10554-CFJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, C-His タグHG10554-CHJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, C-Myc タグHG10554-CMJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, C-HA タグHG10554-CYJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone in cloning vectorHG10554-MJPY18135
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, C-Flag タグHG10554-M-FJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmidHG10554-M-NJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, N-Flag タグHG10554-NFJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, N-His タグHG10554-NHJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, N-Myc タグHG10554-NMJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmid, N-HA タグHG10554-NYJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmidHG10554-UTJPY71331
人々 ENPEP / Aminopeptidase A Gene ORF cDNA clone expression plasmidHG10554-UTJPY71331
 発現ベクターの詳細情報
Product nameProduct name
背景

ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.

参考文献
  • Speth RC, et al. (2008) The significance of brain aminopeptidases in the regulation of the actions of angiotensin peptides in the brain. Heart Fail Rev. 13(3):299-309.
  • Rose JE, et al. (2010) Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Mol Med. 16(7-8):247-53.
  • Pérez I, et al. (2009) Increased APN/CD13 and acid aminopeptidase activities in head and neck squamous cell carcinoma. Head Neck. 31(10):1335-40.
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    カタログ: HG10554-NF
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