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人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグ

  • Human IL12A / IL-12A Gene Plasmid Map 5646
データシートレビュー関連製品プロトコル
人々 VCAM1 cDNA クローン製品情報
Gene_bank_ref_id:NM_001078.2
cDNA サイズ:2241 bp
cDNA の説明:Full length Clone DNA of Homo sapiens vascular cell adhesion molecule 1, transcript variant 1 with N terminal His tag.
遺伝子の別名:VCAM1, CD106, MGC99561, INCAM-100, DKFZp779G2333
:Human
ベクター:pCMV3-SP-N-His
Plasmid:pCMV3-His-VCAM1
制限サイト:KpnI + NotI(6kb+2.24kb)
タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with VCAM1 qPCR primers for gene expression analysis, HP100188 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグ on other vectors
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG10113-ACGJPY59240
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG10113-ACRJPY59240
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10113-CFJPY51990
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His タグHG10113-CHJPY51990
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc タグHG10113-CMJPY51990
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA タグHG10113-CYJPY51990
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone in cloning vectorHG10113-MJPY18140
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10113-M-NJPY51990
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag タグHG10113-NFJPY51990
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His タグHG10113-NHJPY51990
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc タグHG10113-NMJPY51990
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA タグHG10113-NYJPY51990
人々 VCAM1/VCAM-1/CD106 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10113-UTJPY51990
 発現ベクターの詳細情報
Product nameProduct name
背景

Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

参考文献
  • Cook-Mills JM. (2002) VCAM-1 signals during lymphocyte migration: role of reactive oxygen species. Mol Immunol. 39(9): 499-508.
  • Preiss DJ, et al. (2007) Vascular cell adhesion molecule-1: a viable therapeutic target for atherosclerosis? Int J Clin Pract. 61(4): 697-701.
  • Size / Price
    カタログ: HG10113-NH
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    可用性In Stock
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